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Isolation and characterization of two novel groups of kanamycinresistance ColE1like plasmids in Salmonella enterica serotypes from food animals

Description Isolation and characterization of two novel groups of kanamycinresistance ColE1like plasmids in Salmonella enterica serotypes from food animals.

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Isolation and characterization of two novel groups of ~ While antimicrobial resistance in Salmonella enterica is mainly attributed to large plasmids, small plasmids may also harbor antimicrobial resistance genes. Previously, three major groups of ColE1-like plasmids conferring kanamycin-resistance (KanR) in various S. enterica serotypes from diagnostic samples of human or animals were reported. In this study, over 200 KanR S. enterica isolates from .

(PDF) Isolation and characterization of two novel groups ~ Isolation and characterization of two novel groups of kanamycin-resistance ColE1-like plasmids in Salmonella enterica serotypes from food animals Article (PDF Available) in PLoS ONE 13(3):e0193435 .

Characterization of small ColE1-like plasmids conferring ~ Isolation and characterization of two novel groups of kanamycin-resistance ColE1-like plasmids in Salmonella enterica serotypes from food animals Article Full-text available

(PDF) Prevalence of ColE1-Like Plasmids and Kanamycin ~ Isolation and characterization of two novel groups of kanamycin-resistance ColE1-like plasmids in Salmonella enterica serotypes from food animals Article Full-text available

(PDF) CMY-31 and CMY-36 cephalosporinases encoded by ColE1 ~ Isolation and characterization of two novel groups of kanamycin-resistance ColE1-like plasmids in Salmonella enterica serotypes from food animals Article Full-text available

Isolation, characterization, and genetic complementation ~ By using a genetic screen, we have isolated a mammalian cell line that is resistant to infection by retroviruses that are derived from the murine leukemia virus, human immunodeficiency virus type 1, and feline immunodeficiency virus. We demonstrate that the cell line is genetically recessive for the resistance, and hence it is lacking a factor enabling infection by retroviruses. The block to .

Novel plasmid conferring kanamycin and tetracycline ~ These two clusters reflect differences in the genetic makeup, and likely origin, of the plasmids investigated. Download : Download high-res image (1MB) Download : Download full-size image; Fig. 3. A) Full FGD tree, based on ORFeome and amino acid sequences showing two main clusters. The tet(O) clade is indicated separately.

Isolation and characterisation of six novel ~ The effect of pH on phage infectivity was determined for all six phages. After 30 min exposure to pH ranging from 2–11, all six phages were viable between pH 4.0 and pH 10.0, with a 3 log reduction in phage titre occurring at pH 4.0 and pH 10.0 and a 1 log reduction occurring at pH 6.0 and pH 8.0.

Isolation and characterization of novel cDNA clones of ~ Two chitinase and two β-1,3-glucan- . Isolation and characterization of novel cDNA clones of acidic chitinases and β-1,3-glucanases from wheat . plasmids were prepared using the Plasmid Miniprep kit from Qiagen (Valencia, Calif.) and used as templates for sequencing.

Isolation and characterization of novel and short-chain ~ Microorganisms are present everywhere in nature and their cell numbers have been estimated to exceed 10^30. The number of prokaryotes reported in aquatic environments, soil, and subsurface are 10^5 – 10^7 cells/ml, 10^6 – 10^9 cells/g and 10^3 – 10^6 cells/ml, respectively. Interestingly, they are found to have highest density in the human intestine whose cell numbers can exceed 10^11 .

Isolation and characterization of a novel strain of ~ A novel member of extremely halophilic archaea, strain AJ2, was isolated from Ayakekum Lake located in Altun Mountain National Nature Reserve of Xinjiang Uygur Autonomous Region in China. The strain AJ2 requires at least 10% (w/v) NaCl and grows 10% to 30% (optimum at 20%).

Isolation and Characterization of a Novel Immunomodulatory ~ Tricholoma matsutake, a high-class edible mushroom in Japan, has been reported to have excellent biological activities, but difficulty in cultivating the fruit bodies and limited bulk availability have restricted detailed studies. We have developed a method of culturing in tanks, enabling the bulk supply of the mycelia. The preparation (CM6271) exerts modulative effects on the immune .

(PDF) Isolation and characterization of a novel (S ~ Isolation and characterization of a novel (S)-canadine synthase gene from Coptis chinensis Article (PDF Available) in Electronic Journal of Biotechnology 18(5) · September 2015 with 142 Reads

Isolation and Characterization of Canine Natural Killer Cells ~ Samples for the 14 day culture were divided into 3 treatment groups, each containing 1,000 IU/mL of IL-2, 10 ng/mL of IL-15, or 1,000 IU/mL IL-2 + 10 ng/mL IL-15. Samples for the 21 day cultures were grown with EL08-1D2 (EL) feeder cells ( McCullar et al., 2008 ) in media with IL-2 or in cytokine conditions (either IL-2 throughout, IL-2 for 14 .

Prevalence of ColE1-Like Plasmids and Kanamycin Resistance ~ Salmonella strain collection and characterization.. The 102 Kan r Salmonella enterica isolates tested for the presence of ColE1-like plasmids in the present study were collected from samples submitted to veterinary diagnostic laboratories in 2005 in which Salmonella was identified as either the primary or the secondary etiologic agent associated with the illness, as previously described .

Isolation, characterization, and cDNA-derived amino acid ~ 1. Introduction. Members of the phosphagen kinase enzyme family play a key role in the regulation of energy production and utilization in animals (Morrison, 1973, Wyss et al., 1992, Wyss and Kaddurah-Daouk, 2000, Ellington, 2001).These enzymes catalyze the reversible transfer of high-energy phosphoryl groups from ATP to naturally occurring guanidine compounds, producing phosphorylated high .

Isolation and characterization of canine natural killer ~ Fig. 1 shows an example of the cell populations before and after T-cell depletion. On average, 82.7% (range = 76–90%) of the lymphoid cells were CD5 + before depletion, and 10.1% (range 0.9–22.8%) were CD5 + after depletion. The cytokine requirements for in vitro expansion of canine NK cells were unknown. Previous reports of putative canine NK cells suggested these cells express IL-2 .

Isolation and characterization of novel multifunctional ~ Here, we report isolation and characterization of GH26 enzyme from the buffalo rumen metagenome. A novel GH26 gene composed of 1,119 base pairs was successfully amplified using the gene‐specific primers inferred based on the contig generated from metagenome sequence assembly and cloned in a pET32a (+) expression vector as an N‐terminal .

Isolation, characterization and antimicrobial evaluation ~ Results: Structure elucidation by spectral data analysis revealed a novel compound, n-octacosan-7β-ol (OC), yield (0.471%), having significant antimicrobial activity against Leishmania donovani promastigotes, Staphylococcus epidermidis, Escherichia coli, Candida albicans and Aspergillus niger in vitro with GI50 = 5.35, MIC 250, MIC 250 and MFC .

Identification of a Novel Genomic Island Conferring ~ INTRODUCTION. Campylobacter is one of the most common food-borne pathogens worldwide. Two thermophilic species, Campylobacter jejuni and Campylobacter coli, are responsible for most Campylobacter infections in humans. Both C. jejuni and C. coli are prevalent in food-producing animals, and the extensive use of antimicrobials in food animal production has led to an increasing prevalence of .

Cloning of the KanamycinResistance Genefrom Kanamycin ~ Isolation ofakanamycin-resistant transformant. Afterliga-tion of S. kanamyceticus Bcll-cleaved DNA with BglII-cleaved pIJ702, about 10,000 S. lividans Thir transformants were obtained. About 2,000 of these presumptively con-tained recombinant plasmids, since theywereMel-. Oneof these colonies (CMA5)wasfoundto bekanamycinresistant by replication.

Isolation, characterization and chromatography based ~ Download : Download high-res image (127KB) Download : Download full-size image; Fig. 1. Rooted neighbor joining phylogenetic tree of strain rsk5 based on 16S rRNA gene sequences, showing the relationship between strain rsk5 and related representative species of the genus Micrococcus. Numbers at the nodes indicate the levels of bootstrap support .

Isolation, characterization and expression in Escherichia ~ Isolation, characterization and expression in Escherichia coli of a cDNA coding for a novel major wheat food allergen. . We identified a low molecular weight as a novel major wheat food allergen which can be used for the development of component-resolved diagnostic tests for wheat food allergy and eventually for specific immunotherapy.

Isolation and Characterization of Carinactivase, a Novel ~ Isolation of CA-1 and Ecarin. A typical isolation procedure was as follows. One hundred milligrams of lyophilized E. carinatus leucogaster venom were dissolved in 2 ml of 50 mM Tris-HCl, pH 8.0, and insoluble materials were removed by centrifugation. The supernatant was subjected to gel filtration on Superdex 200pg with elution with the same buffer.

2011 Molecular characterization of commensal and ~ plasmids. This study provides a comprehensive understanding of bacteriocins, plasmids and genetic relatedness of commensal and pathogenic E. coli that colonise the human urinary tract and porcine gastrointestinal tract. The bacteriocin analysis revealed differences in its carriage among different groups of E. coli.