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Assessment of eight nucleic acid amplification technologies for potential use to detect infectious agents in lowresource settings
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Assessment of eight nucleic acid amplification ~ 1. PLoS One. 2019 Apr 22;14(4):e0215756. doi: 10.1371/journal.pone.0215756. eCollection 2019. Assessment of eight nucleic acid amplification technologies for potential use to detect infectious agents in low-resource settings.
Assessment of eight nucleic acid amplification ~ Assessment of eight nucleic acid amplification technologies for potential use to detect infectious agents in low-resource settings Jason L. Cantera1, Heather White1, Maureen H. Diaz2, Shivani G. Beall2, Jonas M. Winchell2, Lorraine Lillis1, Michael Kalnoky1, James Gallarda3, David S. Boyle ID 1*
Assessment of eight nucleic acid amplification ~ Nucleic acid amplification technologies (NAATs) are high-performance tools for rapidly and accurately detecting infectious agents. They are widely used in high-income countries to diagnose disease and improve patient care. The complexities associated with test methods, reagents, equipment, quality control and assurance require dedicated laboratories with trained staff, which can exclude their .
Performance and workflow assessment of six nucleic acid ~ Assessment of eight nucleic acid amplification technologies for potential use to detect infectious agents in low-resource settings. Cantera JL, White H, Diaz MH, Beall SG, Winchell JM, Lillis L, Kalnoky M, Gallarda J, Boyle DS. PLoS One, 14(4):e0215756, 22 Apr 2019
Nucleic Acid Isothermal Amplification TechnologiesâA ~ Nucleic acid amplification technologies are used in the field of molecular biology and recombinant DNA technologies. These techniques are used as leading methods in detecting and analyzing a small quantity of nucleic acids.
2011 Automated Real-time Nucleic Acid Amplification 1 ~ Policy statement: automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF system. 1.Tuberculosis, Multidrug-resistant - diagnosis. 2.Tuberculosis - diagnosis. 3.Rifampin -
Nucleic acid amplification technologies - ScienceDirect ~ Nucleic acid amplification technologies Richard D. Abramson and Thomas W. Myers Roche Molecular Systems Inc., Alameda, USA The polymerase chain reaction, Q~ replicase methodology, the ligase chain reaction, the self-sustained sequence replication system, and the new strand displacement assay have continued to progress with the development of improved reagents and new applications.
Nucleic Acid Amplification - an overview / ScienceDirect ~ Nucleic acid amplification (NAA) test is a rapid molecular technique that can detect small quantities of an organism's genetic material in a given specimen. The commonest technique employed is PCR, such as is used in the Xpert (which is a form of NAA test that integrates sample processing and PCR amplification and detection into a single self .
Nucleic Acid Amplification Tests for the Diagnosis of ~ PCR is an attractive tool for diagnosing the cause of pneumonia, because it can detect minute amounts of nucleic acid from potentially all pneumonia pathogens, does not depend on the viability of the target microbe, is probably less affected by previous antimicrobial therapy than are culture-based methods, and provides results quickly.
Nucleic Acid Detection â DFA ~ Isolation, amplification, and detection of DNA and RNA sequences in âmolecular diagnosticâ devices are at the forefront of modern diagnostic medicine.Such technologies offer unprecedented sensitivity and specificity in the detection of infectious disease.Molecular diagnostics are typically very expensive, large, and require a modern laboratory and trained technicians to operate---greatly .
Real-Time Nucleic Acid Sequence-Based Amplification Assay ~ A nucleic acid sequence-based amplification (NASBA) assay in combination with a molecular beacon was developed for the real-time detection and quantification of hepatitis A virus (HAV). A 202-bp, highly conserved 5Ⲡnoncoding region of HAV was targeted. The sensitivity of the real-time NASBA assay was tested with 10-fold dilutions of viral RNA, and a detection limit of 1 PFU was obtained.
(PDF) Nucleic acid sequence based amplification (NASBA ~ Nucleic acid sequence-based amplification (NASBA), an isothermal amplification technique for nucleic acids (NA), was investigated for the species-specific identification of mycobacteria.
David S. Boyle / Environmental & Occupational Health Sciences ~ Assessment of eight nucleic acid amplification technologies for potential use to detect infectious agents in low-resource settings. Performance and workflow assessment of six nucleic acid extraction technologies for use in resource limited settings.
ASMscience / Nucleic Acid Amplificati ~ The development of the polymerase chain reaction, or PCR, by Saiki et al. (1) was a milestone in biotechnology and heralded the beginning of the modern era of molecular diagnostics. Although PCR is the most widely used nucleic acid amplification strategy, other strategies have been developed, and several have clinical utility. These strategies are based on either signal or target amplification.
An Introduction to In Vitro Nucleic Acid Amplification ~ Over the past two decades, the development and optimization of a series of in vitro nucleic acid amplification (NAA) technologies has opened new avenues in diagnostic microbiology for the detection, identification, and characterization of pathogenic organisms [1â3].
ASMscience / Nucleic Acid Amplificati ~ Nucleic acid detection methods play an increasingly important role in the detection of viral infection. This chapter describes the major nucleic acid testing methods and assists in test selection. Nucleic acid amplification methods are classified as target or probe amplification methods based upon the source of the nucleic acid that is amplified in the procedure.
Nucleic Acid Amplification Testing (NAAT) Market / Growth ~ As per the scope of this report, nucleic acid amplification tests (NAAT) are used to detect a particular nucleic acid, virus, or bacteria which acts as a pathogen in blood, tissue, and urine. Nucleic acid amplification tests (NAAT) are thus used to detect HIV RNA or DNA in biological samples in order to diagnose and/or monitor HIV infection.
Nucleic acid test - Wikipedia ~ A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species or subspecies of organism, often a virus or bacteria that acts as a pathogen in blood, tissue, urine, etc. NATs differ from other tests in that they detect genetic materials (RNA or DNA) rather than antigens or antibodies.
Introduction to Nucleic Acid Analysis / LSR / Bio-Rad ~ Nucleic acid analysis generally involves isolation and characterization of the DNA or RNA sample of interest. Sample purification and quality assessment are important steps in experimental workflows since the quality of the recovered nucleic acid can affect the performance in downstream reactions.
Use of Nucleic Acid Amplification Tests in Tuberculosis ~ Nucleic acid amplification tests (NAATs) have been used as a diagnostic tool for tuberculosis (TB) in the United States for many years. We sought to assess NAAT use in TB patients in California during a period of time when NAAT availability increased throughout the world.
Use of Nucleic Acid Amplification Testing for Diagnosis of ~ Nucleic acid amplification testing (NAAT) is the preferred method to detect Chlamydia trachomatis and Neisseria gonorrhoeae, but no commercial tests are cleared by the U.S. Food and Drug Administration for use with extragenital swab samples. This study evaluated the performance of the Gen-Probe Aptima Combo2 assay (Aptima) and the Cepheid Xpert CT/NG assay (Xpert) to detect C. trachomatis and .
Development and evaluation of a realâtime nucleic acid ~ The aim of this study was to develop a realâtime nucleic acid sequenced based amplification (NASBA) using a molecular beacon that could detect a wide range of influenza A subtypes and strains in a single reaction by targeting a conserved region of the influenza genome, and to evaluate its sensitivity and specificity against traditional .
A comparison of nucleic acid amplification techniques for ~ We have investigated the use of PCR, RTâPCR and nucleic acid sequence based amplification (NASBA) for assessment of bacterial viability. The potential to amplify persisting mRNA or DNA targets by the three amplification methods was tested, and results were compared with bacterial viability following heat killing as assessed by a colony count .
Nucleic Acid Amplification Test Quantitation as Predictor ~ Multistep algorithmic testing in which a sensitive nucleic acid amplification test (NAAT) is followed by a specific toxin A and toxin B enzyme immunoassay (EIA) is among the most accurate methods for Clostridium difficile infection (CDI) diagnosis. The obvious shortcoming of this approach is that multiple tests must be performed to establish a CDI diagnosis, which may delay treatment.