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A new synthetic medium for the optimization of docosahexaenoic acid production in Crypthecodinium cohnii

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(PDF) A new synthetic medium for the optimization of ~ A new synthetic medium for the optimization of docosahexaenoic acid production in Crypthecodinium cohnii Article (PDF Available) in PLoS ONE 15(3):e0229556 · March 2020 with 18 Reads

A new synthetic medium for the optimization of ~ A new synthetic medium for the optimization of docosahexaenoic acid production in Crypthecodinium cohnii. PLoS One. 2020; 15(3):e0229556 (ISSN: 1932-6203) Song P; Kuryatov A; Axelsen PH. The heterotrophic microalgae Crypthecodinium cohnii was usually cultivated in complex medium containing glucose, yeast extract and sea salt.

(PDF) Optimization of the production of docosahexaenoic ~ Crypthecodinium cohnii, a heterotrophic marine microalga, is known for accumulating high amounts of docosahexaenoic acid, while offering the advantage of assimilating various carbon sources, such .

(PDF) Repeated fed-batch strategy and metabolomic analysis ~ Background: Docosahexaenoic acid (DHA) is essential for human diet. However, high production cost of DHA using C. cohnii makes it currently less competitive commercially, which is mainly caused by .

Production of docosahexaenoic acid (DHA) by ~ Crypthecodinium cohnii, a marine alga used for the commercial production of docosahexaenoic acid (DHA), was cultivated in medium containing sodium acetate as principal carbon source; the pH was .

Crypthecodinium cohnii with emphasis on DHA production: A ~ A new synthetic medium for the optimization of docosahexaenoic acid production in Crypthecodinium cohnii . During the production of docosahexaenoic acid this algae also produces polysaccharides .

Optimization of docosahexaenoic acid production by ~ Culture conditions of Schizochytrium limacinum SR21 for the purpose of microbial docosahexaenoic acid (DHA) production were investigated. The strain SR21 showed a wide tolerance to salinity; that is, the optimum salinity was between 50% and 200% that of sea water. Monosaccharides (glucose and fructose) and glycerol supported good cell growth and DHA yield.

Crypthecodinium cohnii - an overview / ScienceDirect Topics ~ 7.5.4 Production of Docosahexaenoic Acid (DHA 22:6 n-3) DHA is the largest and most complex of the nutritionally important PUFAs with 22 carbons and 6 methylene-interrupted cis-double bonds. The first-to-market fermentable source of DHA was the heterotrophic microalgae Crypthecodinium cohnii.

Enhanced docosahexaenoic acid production by ~ In this study, date syrup was used for the first time as an alternative carbon source for Crypthecodinium cohnii growth and docosahexaenoic acid (DHA) production by response surface methodology. Four medium variables including date syrup concentration, pH, yeast extract and sea salt concentrations were considered to optimize biomass production.

Optimisation of docosahexaenoic acid production in batch ~ C. cohnii was cultivated in a medium composed of 9 g l −1 glucose, 27.8 g l −1 sea salt and a range of yeast extract concentrations (0–10 g l −1).With increasing yeast extract concentrations the resulting OD values after 50 h growth increased (Fig. 2a).The lipid content of the cells, however, decreased markedly at higher yeast extract concentrations.

Frontiers / Genetic Engineering of Crypthecodinium cohnii ~ In this study, we evaluated suitable selected markers and optimized transformation protocols to develop a new genetic transformation methodology for DHA-producing Crypthecodinium cohnii. Additionally, ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), potentially involved in CO2 fixation under autotrophic conditions, was selected as the target for construction of a gene knockdown mutant.

Optimisation of docosahexaenoic acid production in batch ~ C. cohnii was able to grow on a glucose concentration as high as 84.3 g l-1, although concentrations above 25 g l-1 decreased the growth rate. Comparison of growth at 27 and 30°C showed that the higher incubation temperature was more favourable for growth.

Alternative Carbon Sources for Heterotrophic Production of ~ (A) growth, (B) lipid content of cells, and (C) docosahexaenoic acid (DHA) content of total fatty acids for Crypthecodinium cohnii grown at pH 6.5 in a pH auxostat culture with various concentrations of sodium acetate in the original medium and with additional acetic acid then supplied into the culture on demand. Sodium acetate at (solid circle .

Effects of Nitrogen Source and Vitamin B12 on ~ Abstract. The effects of nitrogen sources and vitamin B 12 on cell growth and docosahexaenoic acid (DHA, C22:6 ω-3) content of Crypthecodinium cohnii ATCC 30556 were investigated. Among the three nitrogen sources (tryptone, yeast extract and corn steep liquor) used, tryptone was the best nitrogen source for C. cohnii.The highest specific growth rate (0.087 h-1) and DHA content (64.34% of .

Docosahexaenoic Acid and Cognition Throughout the Lifespan ~ A new synthetic medium for the optimization of docosahexaenoic acid production in Crypthecodinium cohnii. Song P, Kuryatov A, Axelsen PH. Song P, et al. PLoS One. 2020 Mar 20;15(3):e0229556. doi: 10.1371/journal.pone.0229556. eCollection 2020.

Repeated fed-batch strategy and metabolomic analysis to ~ Docosahexaenoic acid (DHA) is essential for human diet. However, high production cost of DHA using C. cohnii makes it currently less competitive commercially, which is mainly caused by low DHA productivity. In recent years, repeated fed-batch strategies have been evaluated for increasing the production of many fermentation products. The reduction in terms of stability of culture system was one .

Improving docosahexaenoic acid production by ~ Improving docosahexaenoic acid production by Schizochytrium sp. using a newly . Weiwen Zhang, Repeated fed-batch strategy and metabolomic analysis to achieve high docosahexaenoic acid productivity in Crypthecodinium cohnii, Microbial Cell Factories, 10 . Systems and Synthetic Biotechnology for Production of Nutraceuticals, 10.1007/978 .

De novo transcriptomic and metabolomic analysis of ~ First de novo transcriptomic analysis for DHA-producing Crypthecodinium cohnii during fed-batch fermentation. • A total of 82,106 unigenes identified from Crypthecodinium cohnii. • Most up-regulated transcripts enriched in fatty acid and PUFA biosynthesis during late stage of the fermentation. •

Molecules / Free Full-Text / Application of the Response ~ The aim of this study was to determine the cumulative effect of fermentation parameters and enhance the production of docosahexaenoic acid (DHA) by Thraustochytrium sp. ATCC 26185 using response surface methodology (RSM). Among the eight variables screened for effects of fermentation parameters on DHA production by Plackett-Burman design (PBD), the initial pH, inoculum volume, and fermentation .

Fatty Acid Composition and Squalene Content of the Marine ~ Enhanced docosahexaenoic acid production by Crypthecodinium cohnii under combined stress in two-stage cultivation with date syrup based medium. Algal Research 2018, 34, 75-81. DOI: 10.1016/j.algal.2018.07.010. Qiuzhen Wang, Biswarup Sen, Xianhua Liu, Yaodong He, Yunxuan Xie, Guangyi Wang.

Application of Statistically-Based Experimental Designs ~ Abstract. Statistically-based experimental designs were applied to the optimization of medium components and environmental factors for eicosapentaenoic acid (EPA) production by the diatom Nitzschia laevis in heterotrophic conditions. First, the Plackett-Burman design was used to evaluate the effects of variables including medium components and environmental factors on cell growth and EPA .

Altmetric – Optimization of docosahexaenoic acid ~ Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21. . Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21 . 10.1007/s002530051139: Authors: T. Yokochi, D. Honda, T. Higashihara, T. Nakahara View on publisher site Alert me about new mentions. Mendeley readers.

Frontiers / 13C Metabolic Flux Analysis of Enhanced Lipid ~ The heterotrophic microalga Crypthecodinium cohnii has attracted considerable attention due to its capability of accumulating lipids with a high fraction of docosahexaenoic acid (DHA). In our previous study, ethanolamine (ETA) was identified as an effective chemical modulator for lipid accumulation in C. cohnii. In this study, to gain a better understanding of the lipid metabolism and .

Optimization of production of docosahexaenoic acid (DHA ~ Docosahexaenoic acid (DHA) content of mycelium lipid was higher (65–76%) when biomass lipid content was very low (1–2%) and lower (40–50%) when biomass contained a high lipid content (14–18%). DHA yields from glucose, starch and maltose were 270, 325 and 334 mg/L, respectively.

Production of docosahexaenoic and docosapentaenoic acids ~ Vidyah Manikan, Mohd Sahaid Kalil, Aidil Abdul Hamid, Response surface optimization of culture medium for enhanced docosahexaenoic acid production by a Malaysian thraustochytrid, Scientific Reports, 10.1038/srep08611, 5, 1, (2015).