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Decreased nitrite reductase activity of deoxyhemoglobin correlates with platelet activation in hemoglobin Ethalassemia subjects
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The functional nitrite reductase activity of the heme ~ Because IHP lowers hemoglobin oxygen affinity, the authors suggested that hemoglobin oxidation by nitrite was favored by higher oxygen affinity states of hemoglobin. 11,12 Later, in 1981 Doyle et al reported that the reaction of nitrite and deoxyhemoglobin was linearly dependent on hydrogen ion in the pH range of 6.0 to 8.0, however the .
The Reaction between Nitrite and Deoxyhemoglobin ~ F ig.1. The reaction between excess nitrite and deoxyhemoglobin. Deoxyhemoglobin (0.1 m m) was incubated with nitrite (0.5 m m) in a 1-cm path length cuvette under anaerobic conditions, and 80 spectra were taken at 2-min intervals.All spectra were subject to multiple linear regression analysis assuming contributions from deoxyHb, oxyHb, HbNO and metHb, metHb-nitrite, and metHbNO.
Exploring the Mechanisms of the Reductase Activity of ~ The reduction of nitrite to nitric oxide (NO) is a reaction of important physiological consequences that can overcome the decrease in activity of NO synthases under hypoxic conditions. 5 Nitrite reduction by hemoglobin has been proposed to regulate blood pressure, hypoxic vasodilation, platelet activation, and the cellular resilience to hypoxia .
The biochemistry of nitric oxide, nitrite, and hemoglobin ~ In this study, we investigated the nitrite reductase activity of deoxyHb dialysates from 58 non-splenectomized and 23 splenectomized HbE/ß-thal subjects compared to 47 age- and sex-matched normal .
Enhancing the Nitrite Reductase Activity of Modified ~ Enhancing the Nitrite Reductase Activity of Modified Hemoglobin: Bis-Tetramers and their PEGylated Derivatives Francine Evelyn Lui, Doctor of Philosophy, 2011 Department of Chemistry, University of Toronto The need for an alternative to red cells in transfusions has led to the creation of hemoglobin-based oxygen carriers (HBOCs).
Nitrite reductase activity of myoglobin regulates ~ Nitrite represents an endocrine bioavailable storage pool of nitric oxide (NO •) that is bioactivated along the physiological oxygen gradient to regulate a number of vascular and cellular responses (1, 2).Nitrite is present at concentrations of 0.3–1.0 μM in plasma and 1–20 μM in tissue (3–5), and has been proposed to participate in hypoxic vasodilation and signaling (6–9).
Modulating hemoglobin nitrite reductase activity through ~ The production of nitric oxide by hemoglobin (Hb) has been proposed to play a major role in the control of blood flow. Because of the allosteric nature of hemoglobin, the nitrite reductase activity is a complex function of oxygen partial pressure P O2.We have previous developed a model to obtain the micro rate constants for nitrite reduction by R state (k R) and T state (k T) hemoglobin in .
Nitrite Reductase - an overview / ScienceDirect Topics ~ S.C. Feifel, F. Lisdat, in Encyclopedia of Interfacial Chemistry, 2018. Cyt c 551 /cyt c 552 and cytochrome c-nitrite reductase. Loujou et al. have shown bioelectrocatalytic nitrite reduction of a cyt c 551-nitrite reductase (cd 1) from P. aeruginosa via its putative redox partner cyt c 551 (containing one heme group). 122,123 Cyt c 551 and cd 1 NiR have been attached to a PGE by means of a .
(PDF) Increased nitrite reductase activity of fetal versus ~ Reaction of anaerobic deoxy-sHbF and-sHbA with 1 mM nitrite in the absence of dithionite. A and B: disappearance of sheep adult and fetal deoxy-Hb and the formation of iron nitrosyl hemoglobin and .
Nitrite Reductase Activity of Cytochrome c ~ Nitrite reductase activity was measured by mixing reduced cyt c (100 μ m) with lipids (4 m m) and then adding nitrite (5 μ m to 5 m m). All solutions were purged with argon to remove oxygen. Cyt c nitrosylation was monitored by either absorption spectroscopy or EPR. For EPR, aliquots of the samples were frozen as a function of time after mixing.
Deoxymyoglobin Is a Nitrite Reductase That Generates ~ sodilation in vitro1,2 and in vivo1 associated with nitrite reduction by deoxyhemoglobin, consistent with a reaction first characterized by Brooks13 and Doyle14: (1) Nitrite -deoxyHb H 3 NO metHb OH (average k -10.35 mol/L-1 s ) Nitrite is reduced to NO by deoxyhemoglobin, oxidizing hemoglobin in the process. This reaction possesses both pH
Deoxymyoglobin Is a Nitrite Reductase That Generates ~ The bimolecular rate constant of T-state deoxyhemoglobin encapsulated in erythrocytes is reduced by approximately half (0.18 mol/L −1 s −1) in comparison to cell free hemoglobin, because of membrane effects that decrease the rate of nitrite uptake. 1,2 Because of their different reaction rates (Figure 2A) the NO generation profiles for .
Nitrite reductase activity of hemoglobin as a systemic ~ These represent processes in which the allosteric nitrite reductase activity of Hb may make nitrite an ideal therapeutic agent to attenuate the effects of accelerated NO scavenging by cell-free Hb released during intravascular hemolysis (1, 17, 19, 41, 52, 59, 65). In these clinical scenarios, the administration of low-dose nitrite will have .
Enhanced Nitrite Reductase Activity and Its Correlation ~ In addition, hemoglobin bis-tetramers can produce nitric oxide in circulation through their intrinsic nitrite reductase activity. Examination of this activity for hemoglobin-derived bis-tetramers that are acetylated at lysyl amino groups in their α subunits reveals enhanced activity ( k = 2.21 M –1 s –1 ) compared to that of nonacetylated .
Nitrate reductase - Creative Enzymes ~ The NR monomer is composed of a ~100-kD polypeptide, heme-iron, flavin adenine dinucleotide (FAD), and molybdenum-molybdopterin (Mo-MPT). Eight sequence segments of NR are N-terminal "acidic" region, Mo-MPT domain with nitrate-reducing active site, interface domain, hinge 1 containing serine phosphorylated in reversible activity regulation with inhibition by 14-3-3 binding protein, cytochrome .
Myoglobin Is a Nitrite Reductase That Generates NO and ~ Previous studies have demonstrated that nitrite is an endocrine store of NO that may play an important role in hypoxic vasodilation. Nitrite can be converted to NO enzymatically by deoxyhemoglobin, in a reaction that is under allosteric control with a maximum reaction rate near the hemoglobin P 50.In this study, we characterize the nitrite reductase activity of deoxymyoglobin, which reduces .
Nitric oxide formation from the reaction of nitrite with ~ Nitrite is naturally present at low concentrations in vertebrates, where it originates as an oxidative metabolite of nitric oxide (NO) produced by nitric oxide synthases [[]] with some contribution from the diet [[]].In fish, nitrite can also be taken up from the ambient water via active transport across the gills [[]].Recent research has suggested that nitrite constitutes a reservoir of NO .
Nitrite reductase - Wikipedia ~ Assimilatory nitrate reductase is an enzyme of the assimilative metabolism involved in reduction of nitrate to nitrite.The nitrite is immediately reduced to ammonia (probably via hydroxylamine) by the activity of nitrite reductase.. The term assimilatory refers to the fact that the product of the enzymatic activity remains in the organism.
Nitrite reductase activity is a novel function of ~ It followed from these studies that nitrite reductase activity can be attributed to the oxidant site of ubiquinol at the cytochrome bc 1 complex of the respiratory chain (complex III) . Similar to the single electron leak from the respiratory chain to dioxygen, antimycin A was rather stimulating while myxothiazol totally abolished nitrite .
LEAF NITRATE REDUCTASE INDUCTION & ASSAY ~ chloroplast enzyme nitrite reductase (NiR). A complete discussion of the nitrate assimilation pathway can be found in the plant biochemistry text on reserve for the course in the campus library. Low levels of NR exist constitutively, but the majority of the enzyme activity is induced by exposure of plants to nitrate (Lewis, 1986).
Nitrate Reduction to Nitrite, Nitric Oxide and Ammonia by ~ The biological nitrogen cycle involves step-wise reduction of nitrogen oxides to ammonium salts and oxidation of ammonia back to nitrites and nitrates by plants and bacteria. Neither process has been thought to have relevance to mammalian physiology; however in recent years the salivary bacterial reduction of nitrate to nitrite has been recognized as an important metabolic conversion in humans.
Nitrate/Nitrite Toxicity 2013: What Are the Health Effects ~ Hemoglobin molecules contain iron within a porphyrin heme structure. The iron in hemoglobin is normally found in the Fe ++ state. The iron moiety of hemoglobin can be oxidized to the Fe +++ state to form methemoglobin (MetHb). Once it is formed, the molecule loses its ability to carry molecular oxygen and reduces its ability to release oxygen .
Nitrate- and Nitrite-Reductase Activities in Mycobacterium ~ Nitrite reductase activity was greater than nitrate reductase activity. This observation supports the rapid reduction of nitrite and slow reduction of nitrate in M. avium as documented by McCarthy (1987) and explained why M. avium gives a negative result by the standard nitrate reductase test.
Nitrite reductase (NAD(P)H) - Wikipedia ~ In enzymology, a nitrite reductase [NAD(P)H] (EC 1.7.1.4) is an enzyme that catalyzes the chemical reaction. ammonium hydroxide + 3 NAD(P) + + H 2 O ⇌ nitrite + 3 NAD(P)H + 3 H + The 4 substrates of this enzyme are ammonium hydroxide, NAD +, NADP +, and H 2 O, whereas its 4 products are nitrite, NADH, NADPH, and H +.. This enzyme belongs to the family of oxidoreductases, specifically those .