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Perforin Expression Directly Ex Vivo by HIVSpecific CD8 TCells Is a Correlate of HIV Elite Control

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Perforin Expression Directly Ex Vivo by HIV-Specific CD8 ~ Citation: Hersperger AR, Pereyra F, Nason M, Demers K, Sheth P, Shin LY, et al. (2010) Perforin Expression Directly Ex Vivo by HIV-Specific CD8 + T-Cells Is a Correlate of HIV Elite Control. PLoS Pathog 6(5): e1000917.

Perforin Expression Directly Ex Vivo by HIV-Specific CD8 ~ Perforin Expression Directly Ex Vivo by HIV-Specific CD8+ T-Cells Is a Correlate of HIV Elite Control (Article begins on next page) The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters. Citation Hersperger, Adam R., Florencia Pereyra, Martha Nason, Korey

Perforin expression directly ex vivo by HIV-specific CD8 T ~ Citation: Hersperger AR, Pereyra F, Nason M, Demers K, Sheth P, et al. (2010) Perforin Expression Directly Ex Vivo by HIV-Specific CD8 T-Cells Is a Correlate of HIV Elite Control.

Perforin Expression Directly Ex Vivo by HIV-Specific CD8 ~ hiv-specific cd8 t-cells perforin expression directly ex vivo hiv elite control functional correlate viremic controller antigen-specific stimulation chronic progressors polychromatic flow cytometry ex vivo cell inhibitory receptor expression viral load elite controller many immune correlate proliferative ability viremic nonprogressors direct .

HIV-specific CD8 + T cell proliferation is coupled to ~ It is unclear why immunological control of HIV replication is incomplete in most infected individuals. We examined here the CD8+ T cell response to HIV-infected CD4+ T cells in rare patients with .

(PDF) [PROVISIONAL] Perforin Gene PRF1 c.900C>T ~ We observed that HIV-specific CD8(+) T-cells from elite controllers consistently display an enhanced ability to express perforin directly ex vivo compared to all other groups.

HIV-Specific, Ex Vivo Expanded T Cell Therapy: Feasibility ~ HIV-reactive CD8 T cells play a crucial role in the control of HIV viremia.12, 13, 14 However, the existing HIV-specific immune response in ART-suppressed individuals is insufficient to clear persistent infection, even in the presence of LRAs that induce HIV expression.15, 16, 17 Obstacles to immune-mediated clearance of reactivated latent HIV .

CD8 T-Cells from Most HIV-Infected Patients Lack Ex Vivo ~ Hersperger AR, Pereyra F, Nason M, Demers K, Sheth P, et al. (2010) Perforin expression directly ex vivo by HIV-specific CD8 T-cells is a correlate of HIV elite control. PLoS pathogens 6: e1000917. [PMC free article]

Ex vivo T cell–based HIV suppression assay to evaluate HIV ~ To advance T cell–based HIV vaccine development, it is necessary to evaluate the immune correlates of a protective CD8+ T-cell response. We have developed an assay that assesses the capacity ex .

In Vivo Suppression of HIV by Antigen Specific T Cells ~ Abstract. The HIV-specific cytotoxic T lymphocyte (CTL) response is a critical component in controlling viral replication in vivo, but ultimately fails in its ability to eradicate the virus.Our intent in these studies is to develop ways to enhance and restore the HIV-specific CTL response to allow long-term viral suppression or viral clearance.

HIV-Specific, Ex Vivo Expanded T Cell Therapy: Feasibility ~ Sung et al. report the infusion of ex vivo expanded HIV-specific T cells (HXTCs) in antiretroviral-suppressed, HIV-infected individuals was safe and well tolerated. No decrease of the latent HIV reservoir was detected, leaving several challenges ahead, including effective latency reversal and potentially improving in vivo expansion of infused cells.

HIV Controllers Exhibit Effective CD8+ T Cell Recognition ~ Here, we show that ex vivo CD8 + T cells from HIV controllers (possessing at least one HLA-B ∗ 27 or HLA-B ∗ 57 allele), but not HIV progressors expressing the same alleles, are able to directly recognize and kill HIV-infected non-activated CD4 + T cells through the formation of immunologic synapses and class I restricted recognition of .

The simultaneous ex vivo detection of low-frequency ~ The ability to measure antigen-specific T cells at the single-cell level by intracellular cytokine staining (ICS) is a promising immunomonitoring tool and is extensively applied in the evaluation of immunotherapy of cancer. The protocols used to detect antigen-specific CD8+ T-cell responses generally work for the detection of antigen-specific T cells in samples that have undergone at least one .

Ex vivo Detectable Human CD8 T-Cell Responses to Cancer ~ Figure 1. Ex vivo detectable T-cell responses specific for NY-ESO-1, Mage-A10, and Melan-A/Mart-1. After vaccination, PBMCs were collected and analyzed ex vivo by flow cytometry with fluorescent peptide/HLA-A*0201 multimers and CD8-specific mAb.A, numbers indicate percentages of NY-ESO-1, Mage-A10, and Melan-A-specific cells of total CD8 + T-cells. Data are the highest reached percentages .

HIV-specific CD8+ T cells from HIV+ individuals receiving ~ Isolation and expansion of HIV- and CMV-specific CD8 + T-cell clones. All ex vivo manipulations were performed a GMP-facility as previously described. 18,29 CD8 + T cells were stimulated twice in 7-day cycles with 9-11mer peptide (Anaspec)–pulsed adherent cells to obtain several polyclonal CD8 + T-cell lines. For each peptide specificity, lines that both expressed > 2% specific CD8 + T cells .

Control of HIV-1 immune escape by CD8 T-cells expressing ~ Supplementary Figure 2 Kinetic binding analysis of the 868 and a11b6 TCR interactions with HLA A2-SL9. (A) Kinetic binding analysis of the 868 TCR to HLA A2-SL9. An equilibrium binding constant (KD) of 150nM was calculated for the 868 TCR with an association rate (kon) of 1.5 x 10 5 M-1 s-1 and a dissociation rate (k off) of 2.2x10-2 s-1.A range of concentrations were used

CiteSeerX — Ex Vivo Expansion of CD8 CD56 and CD8 CD56 ~ BibTeX @MISC{Wajchman04exvivo, author = {Howard J. Wajchman and Carl W. Pierce and Vijay A. Varma and Et Al and Contact The Aacr and Howard J. Wajchman and Carl W. Pierce and Vijay A. Varma and Muta M. Issa and John Petros and Kenneth E. Dombrowski}, title = {Ex Vivo Expansion of CD8 CD56 and CD8 CD56 Natural Killer T Cells Specific for MUC1 Mucin}, year = {2004}}

PD-1 Expression in HIV-Specific CD8+ T cells Before ~ etween CD8+ T-cell function and phenotype before therapy and HIV persistence on ART. Methods: Blood samples from 29 individuals enrolled during primary HIV infection (at baseline and every 3 months up to 2 years post-ART initiation) were obtained. HIV-specific T-cell function and expression of the activation markers were evaluated before ART by flow cytometry. Cell-associated HIV DNA and .

The Ex Vivo Induction of Human CD103+ CD25hi Foxp3+ CD4 ~ In this study, we demonstrate a novel regulatory role for IL‐2 on TGF‐β1‐mediated ex vivo induction of CD103 + human T cells. In addition, it demonstrates a clear synergistic effect of IL‐2 and TGF‐β1 upon CD103 expression for CD8 + T cells and an additive effect for CD4 + T cells.

HIV Identification and characterization of HIV-specific ~ HIV-specific CD8+ T RMs and effective immune control of HIV. RESULTS CD69+CCR7− CD8+ T cells are expanded in HIV-infected LNs CD69 has often been considered as a marker of early T cell activa-tion (29). We examined CD69 expression on LN CD8 + T cells from HIV+ and HIV− individuals (table S1). Irrespective of adherence to

Impact of HIV on CD8+ T Cell CD57 Expression Is Distinct ~ CD8+ T cells, HIV inhibits this process, expanding less well-differentiated CD28- CD8+ T cells and decreasing the proportion of CD28- CD8+ T cells that express CD57. Citation: Lee SA, Sinclair E, Hatano H, Hsue PY, Epling L, et al. (2014) Impact of HIV on CD8+ T Cell CD57 Expression Is Distinct from That of CMV and Aging.

In Vivo Suppression of HIV by Antigen Specific T Cells ~ The HIV-specific cytotoxic T lymphocyte (CTL) response is a critical component in controlling viral replication in vivo, but ultimately fails in its ability to eradicate the virus. Our intent in these studies is to develop ways to enhance and restore the HIV-specific CTL response to allow long-term viral suppression or viral clearance.

"Impact of HIV on CD8+ T Cell CD57 Expression Is Distinct ~ Methods: We compared markers of CD8+ T cell differentiation (e.g., CD28, CD27, CCR7, CD45RA) and CD57 expression on CD28- CD8+ T cells in healthy HIV-uninfected adults with and without CMV infection and in both untreated and antiretroviral therapy (ART)-suppressed HIV-infected adults with asymptomatic CMV infection.

Microbial exposure alters HIV-1-induced mucosal CD4 + T ~ Early HIV-1 infection causes massive CD4+ T cell death in the gut and translocation of bacteria into the circulation. However, the programmed cell death (PCD) pathways used by HIV-1 to kill CD4+ T cells in the gut, and the impact of microbial exposure on T cell loss, remain unclear. Understanding mucosal HIV-1 triggered PCD could be advanced by an ex vivo system involving lamina propria .

Ex vivo detection of adenovirus specific CD4{sup +} T-cell ~ HIV-infected patients fail to fully recover cell-mediated immunity despite HAART. To identify regulatory factors, we studied the phenotype and function of in vitro cytomegalovirus (CMV)-stimulated T cells from HAART recipients. CFSE-measured proliferation showed CD4{sup +} and CD8{sup +} cells dividing in CMV-stimulated cultures.